Phaseolus Genomics

Home Data WebFPC Overgo Design


PV_GBa User Sequence Submission


*Overgo design and screening of BAC libraries is fraught with difficulties and we don't guarantee success. You should check back periodically to see if your sequence was screened and if it was successful. If your sequence does find a BAC, please order that clone from one of the BAC resource centers such as AGI or CUGI.

1) Not all sequences yield overgos that match our criteria (length, Tm, non-repetitive, etc...).
2) Overgos are, on average, 60-70% successful in finding BACs after hybridization and disambiguation of multi-dimensional data.
3) To exclude any false positives, identified BACs should be confirmed in your lab via Southern or PCR before expending more time or money on them.

Thanks and Good Luck!


Please complete all fields below and click 'Submit'.



First Name:
Last Name:
Email Address:
Organization:
Description of Sequences:
Sequences:


Home Data WebFPC Overgo Design

This work was supported by the National Research Initiative Plant Genome Program of USDA's Cooperative State Research, Education and Extension Service, grant number 2006-03556.